TABLE 1.

Antibiotic, LAT, and clavulanic acid production characteristics of lat::apr mutants

Strain	Total antibiotic concn (μg/ml/OD600)a	LAT activity (U/mg of protein)b	Clavulanic acid titer (μg/ml/OD600)c
Wild type	1.12	1.9	4.5
lat#2	0	0.1	11.7
lat#5	0	NDd	11.5
lat#7	0	ND	11.8
lat#11	0	ND	11.7

^aTotal antibiotic concentrations were determined with supernatants of single cultures grown in TSBS medium for 66 h at 28°C. OD₆₀₀, optical density at 600 nm. 

^bLAT activity was measured with cell extracts prepared from duplicate TSBS cultures grown for 48 h at 28°C. 

^cClavulanic acid titers were determined with supernatants of single cultures grown in SA medium for 66 h at 28°C. 

^dND, not determined.