Figure 2.

TFDG alleviates inflammatory responses in chondrocytes. (a–d) The transcription levels of iNOS, Ptgs2, TNF-α, and IL-6 were determined by RT-qPCR. (e–g) The protein expressions of iNOS and COX2 were analyzed by western blotting. The grey values normalized with β-actin and control group were quantified by ImageJ. (h–k) Proinflammatory cytokines such as PGE2, IL-1β, IL-6, and TNF-α from chondrocytes in different groups detected by ELISA. The bar graph shows the mean ± SD of data (n = 4). ^∗p < .05, ^∗∗p < .01, ^∗∗∗p < .001, and ^∗∗∗∗p < .0001.