FIGURE 4.

Physical contacts of both T cells and MV.Act with HMVECs. (A) FACS representative plots showing VioletTracer‐labelled HCMVECs incubated with CFSE‐labelled activated or nonactivated T cells. Nontreated HCMVECs are shown as a control. Analyses were performed on day 3. Quantitative analysis of CFSE⁺VioletTracer⁺ population in co‐cultures. (B) ImageStream^X imaging flow cytometry of HCMVECs incubated with either activated or nonactivated T cells. Co‐localisation of CFSE⁺ T cell content (green) and VioletTracer⁺ HCMVECs was selectively observed using activated T cells. (C) Flow cytometry plots showing VioletTracer‐labelled HCMVECs incubated with CFSE‐labelled MV.Act. Quantitative analysis of CFSE levels in HCMVECs incubated with MV.Act and control HCMVECs (MFI, mean fluorescence intensity). (D) ImageStream^X imaging flow cytometry of VioletTracer‐labelled HCMVECs incubated with CFSE‐labelled MV.Act. Co‐localisation of CFSE⁺ T cell content (green) and VioletTracer⁺ HCMVECs can be seen (A, C graphs were analysed with the Student's t‐test for two independent groups, *p < .05)