Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Mar 3;40(7):1042–1055. doi: 10.1038/s41587-022-01219-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 6 — a, Mature beta cell signature of SC-beta and adult beta cells from different times of origin. b, Gene sets enriched in the in vivo implanted SC-beta cells upregulated and downregulated genes compared with in vitro SC-beta cells. c, Expression of selected marker genes upregulated in the in vivo SC-beta cells. d, Expression of selected marker genes downregulated in the in vivo SC-beta cells. e, Violin plots representing the expression of mature beta cell markers in the SC-beta cells from S7w0, S7w3 and S7w6 times of origin. f, Average expression of genes associated with mature beta cell hallmark processes in individual SC-beta cell in vitro samples from different times of origin. g, Average expression of glucose metabolism, noncanonical coupling factors and disallowed genes in individual SC-beta cell in vitro samples from different times of origin. h, Immunostaining for disallowed gene LDHA protein and insulin (INS) of in vitro SC-islets from S7w0, S7w3 and S7w6 timepoints. Scale bar, 100 µm. i, Quantification of LDHA positive cells out of all INS positive cells in SC-islets from S7w0, S7w3 and S7w6. Data are presented as mean ± s.e.m. * P < 0.05, ** P < 0.01, *** p < 0.001 One-way ANOVA with Welch’s correction; n = 3. j, Expression of genes associated with insulin secretion and oxidative phosphorylation in SC-beta cells with a high or low mature beta signature. k, Summary of functional and transcriptomic features of SC-islet maturation in vitro and in vivo.