Table 1.
Example of a SLOW5 ASCII file with a single read group
| #slow5_version | 1.0.0 | |||||||
| #num_read_groups | 1 | |||||||
| @asic_id | 0004A30B00232BEC | |||||||
| @exp_start_time | 2020-01-01T00:00:00Z | |||||||
| @flow_cell_id | FAH00000 | |||||||
| @run_id | 855cdb | |||||||
| … | … | |||||||
| #char* | uint32_t | double | double | double | double | uint64_t | int16_t* | … |
| #read_id | read_group | digitisation | offset | range | sampling_rate | len_raw_signal | raw_signal | … |
| read0 | 0 | 8192 | 6 | 1467.6 | 4000 | 123456 | 498,492,… | … |
| read1 | 0 | 8192 | 5 | 1467.6 | 4000 | 2000 | 491,491,… | … |
| … | … | … | … | … | … | … | … | … |
| readN | 0 | 8192 | 3 | 1467.6 | 4000 | 3000 | 400,400,… | … |
A SLOW5 file contains a header (rows with ‘@’ and ‘#’ prefixes) that stores metadata regarding the contents of the file and the ONT experiment(s) contained within, followed by data records (rows with no prefixes) for sequencing reads, with one read per line. SLOW5 format uses tabs (‘\t’) and newlines (‘\n’) as column and row delimiters, respectively. Complete format specifications are provided in Supplementary Note 3.