Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Jul 14;29(7):628–638. doi: 10.1038/s41594-022-00799-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — a, Domain diagrams of human GYS1 and GYG1. Dotted lines represent the construct boundaries of the GYS1–GYG1^ΔCD complex used in all cryo-EM experiments. b, Schematic of the enzyme-catalyzed reactions of GYG1, GYS1 and GBE. Glycogen synthesis is a multistep process consisting of a priming step by GYG followed by an elongation step carried out by GYS and then a branching step by GBE. c, Cryo-EM map and model of the tetrameric GYS1–GYG1^ΔCD complex at 3.0 Å resolution. Individual GYS1 and GYG1 subunits are coloured separately. d, Enlarged view of the GYG1 region interacting with GYS1. GYS1 is coloured purple and GYG1 is coloured coral. e, Residues Cys137, Cys189 and Cys251 form a cysteine-rich pocket on GYS1 at the interface with GYG1. Inset shows different contour levels for the cryo-EM density of Cys137 and Cys189.