Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Jul 14;29(7):628–638. doi: 10.1038/s41594-022-00799-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Extended Data Fig. 1 — a, Coomassie stained SDS-PAGE of small-scale test purifications of GYS1 complexed with differently tagged truncated GYG1. b, Coomassie stained SDS-PAGE of the three GYS1:GYG1 complexes used in this study. c, Blue native PAGE of the three GYS1:GYG1 complexes used in this study. d, Example micrographs of GYS1:GYG1^FL and GYS1:GYG1^ΔCD complexes collected using a Glacios microscope. e, 2D classes of the GYS1:GYG1^ΔCD complex from an initial dataset collected using a Glacios microscope. Arrows indicate regions of fuzzy density protruding from an inter-subunit interface. f, Denaturing mass-spectra of GYS1 and GYG1, as purified and treated with PP1. g, UDP-Glo activity assay of the three GYS1:GYG1 constructs without and with exogenous glycogen. ‘Full’ is the activity assay with all substrates. ‘- Glycogen’ is the assay carried out in the absence of exogenously added glycogen. ‘- Glc6P’ is the assay carried out in the absence of Glc6P. Median and standard deviation of activity is shown (n = 3 technical repeats).

Source data