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. 2022 Mar 3;40(7):1123–1131. doi: 10.1038/s41587-022-01213-5

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 9 — a, Fluorescence over time for Cas13 guides at varying target concentrations (top of each plot, in cp/μL) within the US CDC’s SARS-CoV-2 N1 RT-qPCR amplicon. Compared guides are ADAPT’s design (green), a guide with an active (non-G) PFS at the site of the qPCR probe from the N1 assay (dark gray), and 10 randomly selected guides with an active PFS (light gray). Target concentration of 10⁷ cp/μL is shown in Fig. 5b. b, Fluorescence for Cas13 guides at varying target concentrations within the US CDC’s SARS-CoV-2 N2 RT-qPCR amplicon. The final time point is shown (124 minutes). Guides are as in a. c, Fluorescence over time for Cas13 guides at varying target concentrations (top of each plot, in cp/μL) within the US CDC’s SARS-CoV-2 N2 RT-qPCR amplicon. Guides are as in a.