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Sampling of Corynebacterium culture before and during lysine production. Changes in biomass (●), external glucose (▵), and external lysine (○) were monitored during the course of fermentation of C. glutamicum ATCC 21253 in medium FM4. Samples were withdrawn at time points I (growth phase) and II (growth-and-lysine-production phase) and used for the preparation of total RNA.
