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. 2022 Jul 15;13(7):613. doi: 10.1038/s41419-022-05074-3

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Prefer-fixed sections of 2-month-old WT (A, D, G) and ^rodIgf-1r^−/− (B, E, H) mouse retinas were subjected to immunofluorescence with the IGF-1R (A, B, D, E) and Cre (G, H) antibodies. The sections were imaged at 20× (A–C) and 60× (D–F). C, F, and I These represent the omission of primary antibodies. OS, outer segments; IS, inner segments; ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer. Retinal lysates from IGF-1R-WT and ^rodIgf-1r^−/− mice were immunoblotted with IGF-1R and actin antibodies (J). Densitometric analysis of IGF-1R from whole retinas of WT and ^rodIgf-1r^−/− mice was normalized to actin (K). Data are mean ± SEM (n = 4). An unpaired parametric test with Welch’s correction was used to determine the statistical significance. *p < 0.0006. Tangential serial cryosections from 2-month-old IGF-1R-WT and ^rodIgf-1r^−/− mice were subjected to immunoblot analysis with rhodopsin and IGF-1R antibodies (L). Densitometric analysis of IGF-1R/rhodopsin (M).