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. 2022 Jul 15;13(7):613. doi: 10.1038/s41419-022-05074-3

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — Fatty acid profiles of two-month-old IGF-1R-WT and ^rodIgf-1r^−/− mice were assessed (A–C), as described in the Materials and Methods section. Data are mean ± SEM (n = 3). An unpaired t-test with Welch’s correction was used to determine the significance between these two groups. *p < 0.05. qPCR analysis of fatty acid synthesis genes was performed using retinal cDNA prepared from 2-month-old IGF-1R-WT and ^rodIgf-1r^−/− mouse RNA and the gene expression was normalized to 18S ribosomal RNA (D). Data are mean ± SEM (n = 3). Multiple unpaired t-tests were used to compare the significance between the groups. *p < 0.05. Fatty acid β-oxidation was measured in 2-month-old IGF-1R-WT and ^rodIgf-1r^−/− mouse retinas (E). Data are mean ± SEM (n = 5). Unpaired nonparametric Mann-Whitney test was used to determine the significance. *p < 0.05.