Figure 3.

Reproducibility of observed error rates. Three oligonucleotides synthesized independently using the same synthetic conditions were compared. The synthetic conditions are 1H-tetrazole in anhydrous acetonitrile as an activator, acetic anhydride in THF as a capping reagent A, 10% 1-methylimidazole in 10% pyridine-THF as a capping reagent B, 0.02 M I₂ in THF/pyridine/H₂O (90:<1:10, v/v/v) as an oxidation reagent, and 3% trichloroacetic acid in dichloromethane (TCA) as a deblocking reagent. Q5 High-Fidelity DNA polymerase was used for assembling reaction.