Skip to main content
. 2022 Jul 15;13:4008. doi: 10.1038/s41467-022-31490-2

Fig. 2. Effect of permeating cryoprotective agents on liver function, injury, and microscopic tissue structure after partial freezing at −10 °C for 24 h.

Fig. 2

a Weight gain as percentage of the procurement weight. b Oxygen uptake. c Lactate concentration in the portal vein. d Bile production. e Tissue adenylate energy charge. f Aspartate aminotransferase concentration (AST) in the intrahepatic vena cava (IVC). g Light microscopy images of parenchymal liver wedges at the end of simulated transplantation (×40). Controls (gray) = 1 day hypothermic preservation, GLY glycerol (red), EG ethylene glycol (purple), PG propylene glycol (dark blue). Stars denote statistical significance (two-way ANOVA, followed by Tukey’s post-hoc test): *0.01 < p < 0.05; **0.001 < p < 0.01; ***0.0001 < p < 0.001; ****p < 0.0001. Boxes: floating bars (min to max), with a line at the mean. Source data are provided as a Source Data file.