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. 2022 Jan 20;29(7):1379–1394. doi: 10.1038/s41418-021-00926-5

Fig. 2. H2AZ2 depletion compromises GSC self-renewal/proliferation and tumorigenicity.

Fig. 2

A, B Tumorsphere formation and representative images of GSCs following H2AZ2 KD (N = 6) (mean ± SD). *p < 1.0e−3. C In vitro limiting dilution assays of GSCs transduced with NT/control or H2AZ2 shRNA calculated with ELDA analysis. D qRT-PCR analysis of FABP7 mRNA levels of GSCs with or without H2AZ2 KD (N = 3) (mean ± SD). The housekeeping genes include TBP, TATA box binding protein; HSP70, Heat shock protein 70; and ACTB, beta actin. *p < 0.05. E Transwell migration and invasion assay of GSCs with H2AZ2 KD (n = 3) (mean ± SD). *p < 0.05. F Representative images of (E). G, H In vivo bioluminescence-based imaging 22 days post-orthotropic injection of GSC TS543 (1 × 105 cells) transduced with NT/control or H2AZ2 shRNAs. Quantification of tumor volume based on bioluminescence (G) and representative images of the tumor-bearing mice (H) (n = 5) (mean ± SD). *p < 0.05. I Survival curves of mice implanted with GSC TS543 transduced with NT or H2AZ2 shRNA. A, C–E, G Two-tailed unpaired Student’s t test; I log-rank test.