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. 2022 Jan 20;29(7):1379–1394. doi: 10.1038/s41418-021-00926-5

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© The Author(s), under exclusive licence to ADMC Associazione Differenziamento e Morte Cellulare 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — A Cell viability assay of GSCs and mouse astrocytes with 3 days treatment of S3I-201, E2Fi, or E2Fi/S3I-201 combination at the indicated concentrations (n = 8) (mean ± SD). ***p < 10e−5. B Western blot analysis of cleaved-caspase 3 protein levels with the respective drug treatment of GSC. β-actin serves the loading control. C Gene set enrichment map of pathways containing genes downregulated or upregulated upon 75 μM S3I-201/10 μM E2Fi combination treatment of GSC (3 days). Nodes represent gene sets (pathways) that were significantly enriched in the comparison treated vs. control samples (FDR < 0.01). D Heat map of cell cycle gene expression with DMSO, 10 μM E2Fi, 75 μM S3I-201, and 10 μM E2Fi + 75 μM S3I-201 (3 days) treatment of GSC. E Western blot analysis of p-STAT3, STAT3, E2F1, and H2AZ protein levels with the respective drug treatment of GSC. Vinculin serves the loading control. F Quantification of H2AZ band intensities in (E) when normalized to the vinculin control (n = 3) (mean ± SD). *p < 0.05. G Western blot analysis of H3K27ac and H3 protein levels with the respective drug treatment of GSC. β-actin serves the loading control. H Quantification of H3K27ac band intensities in (G) when normalized to the β-actin control (n = 3) (mean ± SD). *p < 0.05. A, F, H Two-tailed unpaired Student’s t test.