Figure 5.

Engraftment of iPSC-CMs purified using the miR-208a switch-MACS method

Anti-luciferase immunostaining (red) demonstrated that a substantial amount of iPSC-CMs was engrafted in the host mouse left ventricular wall with myocardial infarction three months after the transplantation. Representative data are shown from nine biologically independent experiments. Immunostaining of troponin T (green) marks CMs.

(A) The relatively thin anterior wall of the LV indicates an infarcted area. Scale bar, 1000 μm.

(B) A high magnification of the region of interest indicated by the white square in (A). The troponin T-negative area indicates the scar of the myocardial infarction. Scale bars, 100 μm.