Figure 1.

The HSC compartment can be further subfractionated with CD49b

(A) Fluorescence-activated cell sorting (FACS) profile and gating strategy of phenotypic HSC subsets and further separation with CD49b in CD117-enriched BM cells. Frequencies of parent gates are shown.

(B) In vitro differentiation potential of single sorted cells to myeloid (CD11b⁺Gr-1⁺ and/or CD11b⁺F4/80⁺) and B cells (B220⁺CD19⁺, n_CD49b⁻ = 568 cells, n_CD49b⁺ = 536 cells, n_CD150^int = 401 cells, n_CD150⁻ = 409 cells; nine replicates; five independent experiments). ns, not significant.

(C) Megakaryocyte differentiation culture of single plated cells (n = 360 cells/population, six replicates, three independent experiments).

(D) Erythroid colony-forming assay of CD49b⁻, CD49b⁺, CD150^int, and CD150⁻ cells (n = 8 replicates/population, 30 cells per replicate, two independent experiments). Mean ± SD is shown in (B–D). Statistical significance in (B) was calculated based on total cloning frequency. See also Figure S1.