Figure 2.

CD49b⁻ and CD49b⁺ subsets have different cell-cycle and cell-proliferation kinetics

(A) Cell-cycle analysis of CD117-enriched BM cells. The frequency of G0, G1, and S/G2/M cells in a representative mouse is shown.

(B) Frequency of CD49b⁻ and CD49b⁺ HSCs in G0, G1, and S/G2/M (n = 8 mice, two independent experiments). ns, not significant.

(C) BrdU analysis of CD117-enriched BM cells. The frequency of BrdU⁻ and BrdU⁺ cells in a representative mouse is shown.

(D) Frequency of BrdU⁺ CD49b⁻ and CD49b⁺ HSCs (n = 9 mice, three independent experiments).

(E) Cell divisions from cultured single cells on days 1–4 (n_CD49b⁻ = 347 cells, n_CD49b⁺ = 370 cells, five replicates, three independent experiments). Mean ± SD is shown in (B), (D), and (E).