Figure 3.

CD49b⁻ and CD49b⁺ HSCs are the most durable subsets

(A and B) Total donor contribution (A) and donor contribution to platelets, erythrocytes, and myeloid, B, T, and natural killer cells (B) in peripheral blood (PB) from five cell transplantations (n_CD49b⁻ = 28 mice, n_CD49b⁺ = 22 mice, n_CD150^int = 12 mice, n_CD150⁻ = 13 mice, three independent experiments).

(C) Donor contribution to phenotypic HSCs (LSKFlt-3⁻CD48⁻CD150⁺ or LSKCD48⁻CD150⁺), 5–6 months after five cell transplantations (n_CD49b⁻ = 12 mice, n_CD49b⁺ = 10 mice, n_CD150^int = 12 mice, n_CD150⁻ = 13 mice, three independent experiments). The numbers of reconstituted mice out of all mice analyzed are indicated. ns, not significant. Asterisks indicate statistically significant differences: p = 0.028, CD49b⁻ versus CD150⁻, and p = 0.05, CD150^int versus CD150⁻, month 2; p = 0.032, CD49b⁻ versus CD150⁻, and p = 0.006, CD150^int versus CD150⁻, month 3; p = 0.015, CD49b⁻ versus CD150⁻, and p = 0.005, CD150^int versus CD150⁻, month 4; p = 0.005, CD49b⁻ versus CD150⁻, and p = 0.013, CD150^int versus CD150⁻, month 5; and p = 0.016, CD49b⁻ versus CD49b⁺, month 6. Mean ± SD is shown in (A−C). See also Figure S2.