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. 2022 Jun 16;17(7):1546–1560. doi: 10.1016/j.stemcr.2022.05.014

Figure 5.

Figure 5

CD49b− HSCs are the most durable stem cells

(A) Short-term (ST) or long-term (LT) HSC activity of reconstituted single-cell-transplanted mice (nCD49b− = 28 mice, nCD49b+ = 18 mice, five independent experiments). LT defined as myeloid (M)+ or platelet (P)+ and erythrocyte (Ery)+ in PB, 5–6 months post-transplantation.

(B) Lineage bias distribution of single-cell-transplanted mice with LT activity in (A) (nCD49b− = 25 mice, nCD49b+ = 11 mice, five independent experiments).

(C) Donor contribution to phenotypic HSCs (LSKFlt-3−CD48−CD150+ or LSKCD48−CD150+) 5–12 months after single-cell transplantation (nCD49b− = 27 mice, nCD49b+ = 17 mice, five independent experiments). ns, not significant.

(D) Donor contribution to platelets, erythrocytes, and myeloid, B, T, and natural killer cells in PB from single-cell transplantation (nCD49b− = 10 mice, nCD49b+ = 6 mice, two independent experiments).

(E) Total donor contribution (top) and relative contribution to myeloid, B, T, and natural killer cells (bottom) in PB of CD49b− and CD49b+ single-cell-transplanted mice, 9 months post-transplantation (nCD49b− = 10 mice, nCD49b+ = 6 mice, two independent experiments).

(F) Overview of CD49b− and CD49b+ primary reconstituted single-cell-transplanted mice with LT activity from (A). The categorized lineage bias and number of mice with donor contribution to PB and phenotypic HSCs (LSKFlt-3−CD48−CD150+ or LSKCD48−CD150+) 5–12 months post-transplantation, in primary and secondary transplantation, are indicated.

(G) Donor contribution to phenotypic HSCs (LSKFlt-3−CD48−CD150+ or LSKCD48−CD150+) in secondary transplantation with whole BM cells, 5–6 months post-transplantation (nCD49b− = 50, nCD49b+ = 8 mice, five independent experiments). ns, not significant.

(H) Donor contribution to phenotypic lineage-biased HSC subsets, 5–12 months post-transplantation, from single-cell-transplanted mice (nCD49b− = 27 mice, nCD49b+ = 17 mice, five independent experiments). The number of reconstituted primary donor mice out of all mice analyzed is indicated in (C), (G) and (H). Mean ± SD is shown (C, D, G, H). ns, not significant. See also Figures S4 and S5.