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. 2022 Jun 23;17(7):1620–1635. doi: 10.1016/j.stemcr.2022.05.015

Figure 2.

Figure 2

iAs express canonical astrocytic proteins and perform astrocytic functions

(A–C) Representative maximum-intensity projection immunofluorescence images of GS and glutamate transporters in SAB-HEF-iAs.

(D) Glutamate uptake of rtTA-infected control HEFs, SAB-HEF-iAs, and primary human fetal astrocytes (hAs).

(E) Representative maximum-intensity projection immunofluorescence images of CX43 in SAB-HEF-iAs.

(F) Example immunofluorescence image of SAB-HEF-iAs showing spread of biocytin between S100B+ cells. Arrows indicate cells with biocytin and thick full arrow the injected cell. Individual color levels are adjusted for visibility.

Data are presented as mean ± SEM of n = 4 independent viral transduction experiments at 5 weeks. Kruskal-Wallis test was performed for statistical analysis in (D). ∗p < 0.05. Scale bars, 10 μm (A, B, C, and E) and 25 μm (F). See also Figure S1.