Figure 7.

Improved functionality of induced neurons in co-cultures with iAs

(A) Maximum-intensity projection immunofluorescence images of iNs in co-culture with SB-HEF-iAs (iN + iAs) at 6–8 weeks.

(B) Bright-field images of iN alone and iN + iAs co-cultures.

(C) Example traces of whole-cell current clamp recordings of iNs with three different patterns of action potential (AP) firing. APs are induced by depolarization current step at membrane potential of −70 mV. Current injected in each trace is indicated.

(D) iNs grouped based on number of APs fired.

(E) Example trace of recorded iNs with spontaneous APs at resting membrane potential (RMP). Inset shows magnified view of a single AP.

(F) Percentage of iNs with spontaneous APs at RMP.

(G) Intrinsic membrane properties and AP parameters of iNs.

(H) Example traces of inward Na⁺ and outward K⁺ whole-cell currents evoked by voltage steps ranging from −70 to +40 mV in voltage clamp mode from holding potential of −70 mV.

(I) Outward K⁺ and inward Na⁺ peak currents plotted against voltage steps shown in (H).

Individual color levels are adjusted for visibility in (A). Data are presented as mean ± SEM of n = 38–40 cells from three independent experiments. Unpaired t test was performed for statistical analysis in (G). ^∗p < 0.05. Scale bars, 20 μm (A) and 50 μm (B). See also Figure S7.