FIG. 3.
ARDRA. Shown is a SYBR green II-stained gel (6% acrylamide) of RsaI-digested PCR products amplified with 16S rDNA universal primers (27f and 1492r) from DNA extracted from unamended soil (U) and farmyard manure (FM)- and sewage sludge (SS10 and SS100)-treated plots of Couhins following three different extraction methods: the MS laboratory method (lanes 1 to 3), the MoBio kit method (lanes 4 to 6), and the Bio 101 kit method (lanes 7 to 9). Lanes M, VIII Boehringer Mannheim molecular size markers (sizes indicated in base pairs at left and right). SS10, 10 tons of dry matter/ha/years. SS100, 100 tons of dry matter/ha/2 years.