FIG. 4.

RISA. Shown is SYBR green II-stained gel (6% acryamide) of PCR products amplified with 16S ribosomal universal primers (38_r and 72_f) from DNA extracted from unamended soil (U) and farmyard manure (FM)- or sewage sludge (SS10 and SS100)-treated plots of Couhins soil following three different extraction methods: the MS laboratory method (MS) (lanes 1 to 3), the MoBio kit method (lanes 4 to 6), and the Bio 101 kit method (lanes 7 to 9). Lanes M, VIII Boehringer Mannheim molecular size markers (sizes indicated in base pairs at left and right). Arrow, 800-bp band.