TABLE 3.
Yield of 16S rDNA PCR product amplified from DNA extracted from unamended soil and farmyard manure- and sewage sludge-treated soils following three different extraction methods
| Soila | Yield of 16S rDNA PCR product (arbitrary units) withb:
|
||
|---|---|---|---|
| MS laboratory method | MoBio Laboratories kit | Bio 101 kit | |
| Unamended | 172.5 ± 20.5 a | 194.0 ± 22.6 ab | 223.0 ± 7.7 b |
| Treated with: | |||
| Farmyard manure | 163.4 ± 21.8 a | 163.4 ± 6.9 a | 227.3 ± 9.4 b |
| Sewage sludge (SS10) | 174.5 ± 18.1 a | 192.4 ± 7.5 ab | 218.5 ± 4.5 b |
| Sewage sludge (SS100) | 171.8 ± 21.7 a | 197.8 ± 15.4 ab | 207.0 ± 16.2 ab |
a
SS10, 10 tons of dry matter/ha/yr. SS100, 100 tons of dry matter/ha/2 yr.
b
Values are means ± SE. Values followed by the same letter do not differ significantly (P < 0.05).