Fig. 7.

Nafamostat upregulated the tight junction proteins expression after SCI. A Immunofluorescence image of CD31 (red) and ZO-1 (green) expression in posterior horn of spinal cord at 3 dpi. Scale bar = 50 μm. B, C Quantitative analysis of the area (μm²) of CD31⁺ cells in the images and the fluorescence intensity of ZO-1. D Immunofluorescence image of CD31 (red) and Claudin-5 (green) expression in posterior horn of spinal cord at 3 dpi. Scale bar = 50 μm. E, F Quantitative analysis of the area (μm²) of CD31⁺ cells in the images and the fluorescence intensity of Claudin-5. G Illustration describing the image acquisition area in this figure. Fluorescent photos were acquired at the posterior horn of the spinal cord. Cell nuclei were stained with DAPI (blue). All data represent mean ± SEM. ^nsP > 0.05, *P < 0.05, **P < 0.01, ***P < 0.001 vs. injury (one-way ANOVA with Tukey's post hoc test), n = 3