Fig. 7.

PRDX3 knockdown suppresses the protective effect of HSC-specific YTHDF3 overexpression on liver fibrosis in mice. (A) The YTHDF3 protein level in liver tissues from patients with mild fibrosis (n = 6) or severe fibrosis (n = 8) was analyzed. (B–H) The HSC-specific overexpression of YTHDF3 and PRDX3 shRNA was induced via AAV9-mediated delivery into mice, and the mice were then treated with CCl₄. (B) Dual immunofluorescence staining for the Myc-tagged YTHDF3 and Desmin was performed, n = 3. Scale bar: 200 μm. (C) Immunoblots of Myc-tagged YTHDF3 in primary HSCs isolated from mice after AAV injection, n = 6. (D) H&E, Masson, Sirius Red staining, n = 6. (E) Hydroxyproline content, n = 6. (F) PRDX3, Col1a1, α-SMA proteins, n = 6. (G) TIMP1 and CTGF mRNA levels, n = 6. (H) H₂O₂ content, n = 6. ^#P < 0.05, ^##P < 0.01. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)