Fig. 8.

(GPs’ PCLS): The role of Ca²⁺ and Ca²⁺-sensitisation within PDGF-BB-induced bronchoconstriction. A Inhibition of voltage-gated Ca²⁺-channels by amlodipine: (◆) no pre-treatment/100 nM PDGF-BB (n = 7); (◇) pre-treatment with 100 nM amlodipine/100 nM PDGF-BB (n = 7). B Inhibition of Rho-Kinase by fasudile: (◆) no pre-treatment/100 nM PDGF-BB (n = 5); (◇) pre-treatment with 10 µM fasudile/100 nM PDGF-BB (n = 5). C Inhibition of PKC by calphostin C: (◆) no pre-treatment/100 nM PDGF-BB (n = 5); (◇) pre-treatment with 5 µM calphostin C/100 nM PDGF-BB (n = 5). A–C Statistics was performed by a LMM. p < 0.05 are considered as significant: *p < 0.05, **p < 0.01 and ***p < 0.001