Fig. 7.

ASIC1α regulates the expression of MMP-2 and MMP-9 via the PI3K/AKT/mTOR pathway in HepG2 and SK-Hep1 cells. A1, A2 and A3 Expression levels of MMP-2 and MMP-9 in different group cells were detected by western blotting. The concentration of NVP-BEZ235 was 4 nM and the concentration of MK-2206 was 0.1 µM at 24 h. B1, B2 and B3 Migration abilities of different group cells were examined by wound healing assays. Representative images were taken at × 100 magnification. C1 and C2 Invasion abilities of different group cells were determined by transwell invasion assays. Representative images were taken at × 200 magnification. The concentration of NVP-BEZ235 was 4 nM and the concentration of MK-2206 was 0.1 µM at 24 h. D proliferation abilities of different group cells were determined by MTT assay. The concentration of NVP-BEZ235 was 4 nM and the concentration of MK-2206 was 0.1 µM at 24 h. Data were expressed as the mean ± SD, n = 3. n.s: no significance, *P < 0.05, **P < 0.01 and ***P < 0.001 all versus pH6.5 + NVP-BEZ235 group