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. 2022 Jul 16;20:105. doi: 10.1186/s12964-022-00882-8

Fig. 1.

Fig. 1

The anticancer effects of penfluridol on GBC. A The cell viability measured by the CCK-8 assay of SGC-996, EH-GB1, and GBC-SD cells were treated with PF as indicated for 24 h/48 h. B The colony formation of SGC-996, EH-GB1, and GBC-SD cells were seeded in 6-well plates and treated with PF as indicated for 14 days. C Annexin V/PI staining apoptosis assay of SGC-996, EH-GB1, and GBC-SD cells treated with PF as indicated for 24 h. D The Western blotting of SGC-996, EH-GB1, and GBC-SD were treated with PF as indicated for 24 h. E Cell cycle assay of SGC-996, EH-GB1, and GBC-SD cells treated with PF as indicated for 24 h. F The Western blotting of SGC-996, EH-GB1, and GBC-SD were treated with PF as indicated for 24 h. *P < 0.5, **P < 0.01, ***P < 0.001, ****P < 0.0001