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. Author manuscript; available in PMC: 2022 Aug 1.
Published in final edited form as: Nature. 2022 Apr 28;606(7914):585–593. doi: 10.1038/s41586-022-04802-1

Figure 2. SARS-CoV-2 replicates in human macrophages.

Figure 2.

a. Representative flow cytometry plots and frequencies of mNG+ human (CD68+) or mouse (F4/80+) lung macrophages in SARS-CoV-2-mNG infected MISTRG6-hACE2 mice. Human n=7, mouse n=6 mice over at least 3 experiments. Unpaired, two-tailed t-test.

b. Quantification of gRNA and sgRNA (E-gene)40,69 in sorted mNG+ or mNG− epithelial cells or human immune cells. N=3 mice over 2 experiments. Means with datapoints.

c. Representative fluorescent microscopy images of dsRNA (rJ2), CD68 and DAPI staining in fixed lung tissues from SARS-CoV-2 infected MISTRG6-hACE2 mice. Representative of n=5 mice examined over 3 experiments. Yellow rectangle: higher magnification view of the selected area. Yellow arrow: colocalization of CD68 with dsRNA. Pseudo-colors were assigned.

d. Representative fluorescent microscopy images of RdRp, CD68 and DAPI staining in fixed lung tissues from SARS-CoV-2 infected MISTRG6-hACE2 mice. Representative of n=5 mice examined over 3 experiments. Yellow arrows: colocalization of human CD68 with dsRNA. Yellow rectangle: higher magnification view of the selected area. Pseudo-colors were assigned.

e. Frequencies of mNG+ human immune cells in Remdesivir treated (1–3dpi) or control MISTRG6-hACE2 mice infected with SARS-CoV-2-mNG. N=6 mice examined over 3 experiments. Means with datapoints. Paired t-test, two-tailed.

f. Frequencies of mNG+ human immune cells upon ACE2 blockade (1–3dpi) in MISTRG6 (no AAV) mice infected with SARS-CoV-2-mNG. CTRL-infected n=5, anti-ACE2 treated n=6 mice examined over 2 experiments. Means with datapoints. Paired t-test, two-tailed.

g. Representative flow cytometry plots and frequencies of mNG+ macrophages in infected MISTRG6-hACE2 mice treated with monoclonal antibodies (mAb)19,45,64 at 35hpi. CTRL-infected n=7, treated n=4 mice examined over 2 experiments. Means with datapoints. Unpaired, two-tailed t-test.

h. Frequencies of mNG+ human immune cells in MISTRG6-hACE2 mice after CD16 blockade (Abcam, 2dpi). N=6 mice examined over 3 experiments. Means with datapoints shown. Paired t-test, two-tailed.