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. Author manuscript; available in PMC: 2022 Aug 1.
Published in final edited form as: Nature. 2022 Apr 28;606(7914):585–593. doi: 10.1038/s41586-022-04802-1

Extended data figure 4. Therapeutics reduced expression of a representative list of interferon stimulated genes- ISGs (DDX58, IFIH1, IFITM3, IRF7) or inflammatory markers (IL6) (matched to figure 1).

Extended data figure 4.

Relative expression of interferon inducible or inflammatory genes in treated or untreated MISTRG6- hACE2 mice infected with SARS-CoV-2 mice at 14dpi or 28dpi. Uninfected baseline expression values are presented as reference. The distribution of cells that preferentially express these genes is overlayed on the tSNE plots showing 14dpi and 28dpi human immune cells. IFITM3 and IL6 were particularly enriched in macrophage/monocyte clusters, while IRF7, DDX58 and IFIH1 were enriched in multiple immune cells such as T cells, B cells, and myeloid cells. Normalized to HPRT1. DDX58: uninfected n=3; CTRL-infected: 14dpi n=8, 28dpi n=: 5; Dex 14dpi n=3, 28dpi n= 6; RDV 14 and 28dpi n=3; anti-IFNAR2 14 and 28 dpi n=3, anti-IFNAR2+ Remdesivir 14 and 28dpi n=5 biologically independent mice examined over at least 2 independent experiments. IFIH1: uninfected n=5; CTRL-infected: 14dpi n =11, 28dpi n=: 3; Dex 14 and 28dpi n=4; RDV 14 and 28dpi n=3; anti-IFNAR2 14 and 28 dpi n=3, anti-IFNAR2+ Remdesivir 14 dpi n=4 and 28dpi n=5 biologically independent mice examined over at least 2 independent experiments. IFITM3: uninfected n=4; CTRL-infected: 14dpi n =7, 28dpi n=: 4; Dex 14 and 28dpi n=4; RDV 14 and 28dpi n=3; anti-IFNAR2 14 and 28 dpi n=3, anti-IFNAR2+ Remdesivir 14 and 28dpi n=4 biologically independent mice examined over at least 2 independent experiments. IRF7: uninfected n=4; CTRL-infected: 14dpi n =7, 28dpi n=: 5; Dex 14 and 28dpi n=4; RDV 14 and 28dpi n=3; anti-IFNAR2 14 and 28 dpi n=3, anti-IFNAR2+ Remdesivir 14dpi n=4, 28dpi n=5 biologically independent mice examined over at least 2 independent experiments. IL6: uninfected n=5; CTRL-infected: 14dpi n =9, 28dpi n=: 4; Dex 14dpi n=3, 28dpi n=4; RDV 14 and 28dpi n=3; anti-IFNAR2 14 and 28 dpi n=3, anti- IFNAR2+ Remdesivir 14dpi n=4, 28dpi n=5 biologically independent mice examined over at least 2 independent experiments. Unpaired, two-tailed t-test.