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. 2022 Jul 16;25(8):104766. doi: 10.1016/j.isci.2022.104766
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

Goat anti-human IgG, 1:4000 Jackson 109-035-098; RRID: AB_2337586
Goat anti-human IgA, 1:750 Thermo Fisher Scientific 31417; RRID: AB_228253
Goat anti-human IgM, 1:3,000 Sigma-Aldrich A6907; RRID: AB_258318
Mouse anti-human IgG1, 1:3,000 SouthernBiotech 9054–05; RRID: AB_2796627
Mouse anti-human IgG2, 1:3,000 SouthernBiotech 9060–05; RRID: AB_2796633
Mouse anti-human IgG3, 1:3,000 SouthernBiotech 9210–05; RRID: AB_2796699
Mouse anti-human IgG4, 1:3,000 SouthernBiotech 9200–05; RRID: AB_2796691
Mouse anti-human IgG4, 1:500 Invitrogen A-10654; RRID: AB_2534054

Chemicals, Peptides, and Recombinant Proteins

spike ECD (for ELISA), HEK293, 1 μg/mL (Emmenegger et al., 2020), Oxford N/A
WT spike S1 (for ELISA), HEK293, 1 μg/mL AcroBiosystems S1N-C52H2
WT spike S2 (for ELISA), HEK293, 1 μg/mL AcroBiosystems S2N-C52H5
WT RBD (for ELISA), HEK293, 1 μg/mL (Emmenegger et al., 2020), Oxford N/A
WT RBD (for MAAP), HEK293, 1 μg/mL Sino Biological 40592-V08H
Delta RBD (for ELISA, MAAP), HEK293, 1 μg/mL Sino Biological 40592-V08H90
Omicron RBD (for ELISA, MAAP), HEK293, 1 μg/mL Sino Biological 40592-V08H121
NC (for ELISA), HEK293, 1 μg/mL AcroBiosystems NUN-C5227
Purified IgA (for ELISA), Human serum, 2.5 μg/mL Sigma-Aldrich 14036
Purified IgM(for ELISA), Human serum, 2.5 μg/mL Sigma-Aldrich 18260
Recombinant human IgG1 (for ELISA), HEK293, 2.5 μg/mL Mabylon AG N/A
Recombinant human IgG2 (for ELISA), Human myeloma, 2.5 μg/mL EMD Millipore AG504; RRID: AB_97839
Recombinant human IgG3 (for ELISA), Human myeloma, 2.5 μg/mL EMD Millipore AG506; RRID: AB_97840
Recombinant human IgG4 (for ELISA), HEK293, 2.5 μg/mL Mabylon AG N/A