TABLE 1.
Strains and plasmids
| Strain or plasmid | Relevant characteristic(s) | Reference or source |
|---|---|---|
| Strains | ||
| A. irakense | ||
| KBC1 | Wild type; LMG 10653; isolated from rice rhizosphere | 11 |
| FAJ0694 | KBC1 celA::kan Kmr | This work |
| E. coli DH5α | F80dlacZΔM15 Δ(lacZYA-argF) recA endA hsdr supE | Gibco BRL |
| Plasmids | ||
| pUC19 | Cloning vector; Apr | 34 |
| pBluescript SK(−) | Cloning vector; Apr | Stratagene |
| pSUP202 | Mobilizable plasmid; suicide vector for A. irakense; Cmr Tcr Apr | 24 |
| pRK2013 | Tra+ helper plasmid | 9 |
| pLAFR1 | IncP1 broad-host-range plasmid;Tcr | 10 |
| pLAFR3 | pLAFR1 derivative containing the pUC8 multiple cloning site | 26 |
| pHP45Ω-Km | Tcr; contains Kmr cassette | 8 |
| pFAJ0649 | pLAFR1 derivative from genomic library of A. irakense containing celA | This work |
| pFAJ0680 | pBluescript SK(−) with 5.5-kb EcoRI insert from pFAJ0649; expresses CelA | This work |