Strains |
A. irakense
|
KBC1 |
Wild type; LMG 10653; isolated from rice rhizosphere |
11 |
FAJ0694 |
KBC1 celA::kan Kmr
|
This work |
E. coli DH5α |
F80dlacZΔM15 Δ(lacZYA-argF) recA endA hsdr supE
|
Gibco BRL |
Plasmids |
pUC19 |
Cloning vector; Apr
|
34 |
pBluescript SK(−) |
Cloning vector; Apr
|
Stratagene |
pSUP202 |
Mobilizable plasmid; suicide vector for A. irakense; Cmr Tcr Apr
|
24 |
pRK2013 |
Tra+ helper plasmid |
9 |
pLAFR1 |
IncP1 broad-host-range plasmid;Tcr
|
10 |
pLAFR3 |
pLAFR1 derivative containing the pUC8 multiple cloning site |
26 |
pHP45Ω-Km |
Tcr; contains Kmr cassette |
8 |
pFAJ0649 |
pLAFR1 derivative from genomic library of A. irakense containing celA
|
This work |
pFAJ0680 |
pBluescript SK(−) with 5.5-kb EcoRI insert from pFAJ0649; expresses CelA |
This work |