Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Jun 25;144(2):305–337. doi: 10.1007/s00401-022-02452-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 6 — Anti-OPN antibody treatment neutralizes OPN in peri-infarct NVU cells in mice post-acute ischemic stroke. a–d Representative images of immunofluorescence staining for OPN (red) and cell-specific markers (green) including podocalyxin for endothelial cells (a), CD13 for pericytes (b), GFAP for astrocytes (c) and IBA1 for microglia/macrophages (d) in the peri-infarct region (left) and contralateral hemisphere (right) of Ctrl IgG and α-OPN antibody-treated mice 24 h post-stroke. White arrowheads indicate OPN expressing NVU cells. e–h Quantification of OPN expression in peri-infarct and contralateral endothelial cells (e), pericytes (f), astrocytes (g) and microglia/macrophages (h) utilizing 3 images/region/animal, n = 12 and 10 for Ctrl and α-OPN, respectively. */§P < 0.05, **/§§P < 0.01, ***P < 0.001, §§§§P < 0.0001 and ns P > 0.05. *Two-tailed, unpaired t test, with Welch’s correction when variances were significantly different based on F test, comparing the two treatments groups for the same region, and ^§two-tailed, paired t-test comparison of the peri-infarct and contralateral hemisphere within the same treatment group/animal. Scale bars: 10 µm and 5 µm in insets (a–d)