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Determination of the sensitivity of amplification by RT-PCR of the BQCV. BQCV virus stock containing 1.3 × 108 genome copies/μl was serially 10-fold diluted. One microliter of this stock was used in the RT-PCR mixture. M, Pst lambda DNA marker; lane 1, BQCV; lane 2, 10−1 BQCV dilution; lane 3, 10−2 BQCV dilution; lane 4, 10−3 BQCV dilution; lane 5, 10−4 BQCV, lane 6, 10−5 BQCV; lane 7, 10−6 BQCV dilution; lane 8, 10−7 BQCV dilution; lane 9, water (negative control).
