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. 2022 Jul 16;13:4143. doi: 10.1038/s41467-022-31865-5

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a Confocal micrographs (maximum intensity projections) of Leptomycin B, Selinexor and control DMSO treated U2OS cells, stained for CIP2A. b Quantification of nuclear CIP2A staining of the experiment in a. Each data point corresponds to one cell (DMSO: n = 173, LeptomycinB: n = 150, Selinexor: n = 178, pooled from two independent experiments). Bars and error bars represent mean and SD. Statistical significance was calculated using one-way ANOVA and Dunnett’s multiple comparison test (α = 0.05). c Confocal micrographs (maximum intensity projections) of RPE-1 ΔCIP2A cells and RPE-1 ΔCIP2A cells stably transduced with Flag-tagged full-length CIP2A (WT) and CIP2A deletion mutant (human CIP2A amino acids 561–625; ΔNES), stained for CIP2A. d Quantification of nuclear CIP2A staining of the experiment in c. Each data point corresponds to one cell (+Epmpty: n = 199, +Flag-WT: n = 155, +Flag-ΔNES: n = 165, pooled from two independent experiments). Bars and error bars represent mean and SD. Statistical significance between +Flag-WT and +Flag-ΔNES was calculated using two-sided Welch’s t-test (α = 0.05). e Flag-immunoprecipitation from 293FT cells either Mock transfected (Mock) or transfected with a Flag-tagged full-length CIP2A wild type (WT), and Flag-tagged CIP2A lacking amino acids 561-625 (ΔNES). Relative intensities of co-immunoprecipitated CRM1 bands are indicated. f Confocal micrograph (maximum intensity projection) of Hela cells expressing endogenous Clover-tagged LMNA and stained for CIP2A. g Quantification of CIP2A-TOPBP1 proximity by in situ PLA in U2OS cells transfected with control siRNA (siCtrl) and siRNA against TOPBP1 (siTOPBP1), arrested in mitosis by Nocodazole and treated with 1 Gy of IR. Interphase cells (I) were separated from mitotic cells (M) with an automatic image analysis pipeline, based on DAPI mean intensities. Each data point represents one cell (siCtrl I: n = 92, siCtrl M: n = 63, siTOPBP1 I: n = 138, siTOPBP1 M: n = 121; pooled from two independent experiments), and bars represent median. Statistical significance was assessed by Kruskal-Wallis test and Dunn’s multiple comparison test (α = 0.05). Source data are provided as a Source Data file.