a-c Relative mRNA level of different genes in the liver from WT and Gm4951−/− mice on chow diet (a) and HFD (b) for four weeks (n = 3 mice per genotype), and chow diet for 24 weeks (c) (n = 5 mice per genotype). Levels were normalized to Polr2a mRNA and then to levels in WT mice. d Relative mRNA level of different genes in hepatocytes from WT and Gm4951−/− mice after 0.5 mM OA for 24 h (n = 3 mice per genotype). e, f CO2 released (e) and acid soluble CoAs (f) from fatty acid oxidation experiment were measured with scintillation counting and normalized with total protein of liver lysates (n = 5 mice per genotype). g The serum level of triglycerides at different time points after Triton WR-1399 injection in WT and Gm4951−/− mice on HFD for 4 weeks (n = 3 mice per genotype). h Relative mRNA level of Gm4951 in the liver from C57BL6/J mice on chow diet or HFD for 24 weeks (n = 3 mice per genotype). Levels were normalized to Polr2a mRNA and then to levels in WT mice fed chow diet. i Relative mRNA level of 3xFlag-Gm4951 in the liver from mice of the indicated genotypes on chow diet at eight weeks of age (n = 4 mice per genotype). n.d. not detected. Levels were normalized to Polr2a mRNA and then to levels in WT mice. Each data point represents one mouse (a–f, h–i). Data are presented as means ± SD. P values were determined by two-tailed Student’s t test. P values are denoted by *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.001; ns, not significant with P > 0.05. The exact P values of statistically significant groups are: 0.0176 (b, Ppara); 0.0009 (b, Acox1); 0.0418 (c, Apob); 0.0041 (c, Mttp); 0.0281 (c, Ppara); 0.0017 (d, Ppara); 0.0401 (d, Acot1); 8.466 × 10−5 (d, Acox1); 0.0288 (d, Acadl); 0.0201 (e); 0.0039 (f); 0.0116 (g, 0 h); 0.0132 (g, 1 h); 0.0040 (g, 2 h); 0.0005 (h); 6.292 × 10−6 (i). Data are representative of two independent experiments (a–d, g–i) or from one experiment (e–f).