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. Author manuscript; available in PMC: 2023 Aug 1.
Published in final edited form as: J Pineal Res. 2022 Jun 5;73(1):e12808. doi: 10.1111/jpi.12808

Figure. 4. Impact of MLT and AGP on β-catenin and Rho-activity in HT29-s.

Figure. 4.

The impact of MLT and AGP on luciferase reporter activity. Spheroids were treated with or without AGP/MLT as previously described. The results are presented as ratio of firefly luminescence to Renilla luminescence. The y-axis represents the luciferase activity of treated colospheroids relative to untreated spheroids (**P< 0.01, ***P<0.001). B. A pull-down assay was used to detect RhoA activity as described. Quantification of relative phospho-Rho kinase level/total Rho kinase by densitometry measurements of immunoblots from three independent experiments. (**P<0.01, ***P<0.001).