Figure 6. MLT and AGP synergistically downregulates angiogenic signaling in colospheroids.
Treated/untreated HT29-s lysates were subjected for protein expression as indicated. Phospho protein was normalized with total protein B-D. All statistically significant differences were determined using one-way ANOVA followed Bonferroni test (*P<0.05, **P< 0.01, ***P<0.001). D, F-G. MLT+AGP suppressed angiogenic branching and total capillary tube length. E. Matrigel invasion inhibition of HT29-s after treatment as indicated. H. The invaded cells were quantified by counting using an optical microscope at 100X magnification and averaged after counting five fields per membrane (**P< 0.01, ***P<0.001).