Craig's medium 15% polyacrylamide gel after silver nitrate staining. In all four columns for the concentrated toxin samples, the 11.6 and 28 kDa bands were observed for the B and A subunits of cholera toxin, respectively. B = BSA, M = Protein marker, D1 = 1:5, D2 = 1:10, D3 and D4 = 1:100 Toxin dilutions.