Table 2. Relation of restriction endonucleases, temperature and expected fragments.

Gene	Restriction endonucleases	Restriction profile	Temperature (°C)	Fragments
IL-1A−889	NcoI	5′-C/CATGG-3′ 3′-GGTAC/C-5′	37 °C	83 bp + 16 bp (allele 1) 99 bp (allele 2)
IL-1B+3954	Taql	5′-T/CGA-3′ 3′-AGC/T-5′	65 °C	12 bp + 85 bp+ 97 bp (allele 1) 12 bp +182 bp (allele 2)