TABLE 3.
Primers used in this study
| Gene(s), primers, and sequences (5′ to 3′) | Amplicon size (bp) | PCR conditionsa | Source or reference; accession no. |
|---|---|---|---|
| ctxA and ompU (CT subunit A and outer membrane protein) | 60–1 | ||
| 94F, CGG GCA GAT TCT AGA CCT CCT G | 564 (ctxA) | 19 | |
| 614R, CGA TGA TCT TGG AGC ATT CCC AC | |||
| 80F, ACG CTG ACG GAA TCA ACC AAA G | 869 (ompU) | This study; AE004149, AF253529, U73751 | |
| 906R, GCG GAA GTT TGG CTT GAA GTA G | |||
| zot and toxR (zonula occludens toxin and operon ToxR) | 60–1 | ||
| 225F, TCG CTT AAC GAT GGC GCG TTT T | 947 (zot) | This study; AE004224, AF123049, AF175708, M8363 | |
| 1129R, AAC CCC GTT TCA CTT CTA CCC A | |||
| 101F, CCT TCG ATC CCC TAA GCA ATA C | 779 (toxR) | This study; M21249, AE004179 | |
| 837R, AGG GTT AGC AAC GAT GCG TAA G | |||
| tcpA (TCP A [Classical and El Tor]) | 60–1 | ||
| 72F, CAC GAT AAG AAA ACC GGT CAA GAG | 451 (El Tor) | Modified from reference 39; AE004168, UO9807, X64098, X09807 | |
| 477R, CGA AAG CAC CTT CTT TCA CGT TG | |||
| 647R, TTA CCA AAT GCA ACG CCG AAT G | 620 (Classical) | ||
| tcpI (TCP I) | 60–3 | ||
| 132F, TAG CCT TAG TTC TCA GCA GGC A | 862 (tcpI) | This study; AE004168, L25659, X64098 | |
| 951R, GGC AAT AGT GTC GAG CTC GTT A | |||
| hlyA (hemolysin [Classical and El Tor]) | 60–1 | ||
| 489F, GGC AAA CAG CGA AAC AAA TAC C | 481 (El Tor) | Modified from reference 69; AE004362, AF117834, M36855, X51746, Y00557 | |
| 744F, GAG CCG GCA TTC ATC TGA AT | 738/727 (ET/Clas) | ||
| 1184R, CTC AGC GGG CTA ATA CGG TTT A | |||
| stn/sto (non-O1 heat-stable enterotoxin) | 55–1 | ||
| 67F, TCG CAT TTA GCC AAA CAG TAG AAA | 172 (stn/sto) | This study; M85198, X74108 | |
| 194R, GCT GGA TTG CAA CAT ATT TCG C |
a
Temperature of annealing (°C)–time of extension (min).