Figure 3.

Expression and processing of Spike proteins containing mutations present in the Omicron BA.1 and BA.2 VOCs

(A) The upper panels show exemplary immunoblots of whole cells lysates (WCLs) and VSVpp containing supernatants of HEK293T cells transfected with vectors expressing the Hu-1, BA.1, BA.2, or mutant SARS-CoV-2 S proteins and infected with VSVΔG-GFP. Blots were stained with anti-V5 (Spike), anti-ß-actin, and anti-VSV-M protein. Lower panels: expression levels of uncleaved, full-length Spike protein (S, gray bars) and the S2 subunit (bars colored according to the corresponding domains, as shown in Figure 1D) were quantified. The results show mean values (±SEM) obtained from three independent experiments.

(B) Correlation of the S2 expression/incorporation and S2/S processing of the parental S Hu-1 or indicated mutant S proteins in cells and supernatants, with the corresponding pseudotype infection rates. The correlation coefficients ( r values) and two-tailed p values are provided.

See also Figure S2.