Fig. 4.

Monocyte and DC subsets from older individuals are impaired in TANK-binding kinase (TBK) phosphorylation. Human PBMCs from the blood of younger (age, 24–36 years; n = 11) and older (age, 67–83 years; n = 11) healthy donors were either stimulated with LPS/IFN-γ, G10, a cGAS-STING-specific agonist, or transfected with a RIG-I-specific 3p-hpRNA ligand. a Representative flow plot for data presented in b–d. Using these plots, the fold change of pTBK-1 MFI was calculated using the unstimulated as the control. b–d PBMCs were either stimulated with LPS/IFN-γ, G10, or the RIG-I agonist or left unstimulated for 15 min and 24 h. Cells were permeabilized, fixed, and stained for phosphorylated TBK-1. After gating on DC and monocyte subsets, the MFI was measured using Phosflow cytometry. Data are means. *p < 0.05, **p < 0.01, non-parametric Mann–Whitney U test