Fig. 5.

Monocyte and DC subsets from older individuals are impaired in cGAS-STING activation. Human PBMCs from the blood of younger (age, 24–36 years; n = 11) and older (age, 67–83 years; n = 11) healthy donors were either stimulated with LPS/IFN-γ, G10, a cGAS-STING-specific agonist, or transfected with a RIG-I-specific 3p-hpRNA ligand. a Representative flow plot for data presented in b–d. Using these plots, the fold change of STING MFI was calculated using the unstimulated as the control. b–d PBMCs were either stimulated with LPS/IFN-γ, G10, or the RIG-I agonist or left unstimulated for 15 min and 24 h. Cells were permeabilized, fixed, and stained for STING. After gating on DC and monocyte subsets, the median intensity (MFI) was measured using flow cytometry. Data are means. *p < 0.05, **p < 0.01, p < 0.001, non-parametric Mann–Whitney U test