Fig. 6.

Phagocytosis in response to pathway-specific stimulus is impaired in multiple innate immune subsets from older adults. a–e Phagocytosis is less efficient in cDC1 (b), cDC2 (c), CD14^dimCD16⁺ monocytes (d), and CD14⁺CD16.⁻ monocytes (e). Phagocytosis was measured using fluorescent beads and multiparametric flow cytometry. PBMCs were incubated overnight with a stimulus and with beads for a further 3-h period. MFI of fluorescent beads of indicated subsets from young (n = 11) and old (n = 11) patients is indicated. Data is from two independent experiments and are shown (mean ± SEM). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 The p values were determined using the non-parametric Mann–Whitney U test