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. 2022 Jul 4;12:920444. doi: 10.3389/fonc.2022.920444

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Copyright © 2022 Wanigasooriya, Barros-Silva, Tee, El-asrag, Stodolna, Pickles, Stockton, Bryer, Hoare, Whalley, Tyler, Sillo, Yau, Ismail and Beggs

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Genome-wide CRISPR screens identify mTOR signalling up-regulates pathway genes. (A) Volcano plot depicting significantly differentially expressed genes (p < 0.05 and |fold change| > 1) in red. Model-based analysis of genome-wide CRISPR-Cas9 knockout on libraries A and B detected twenty-six expressed genes from mTOR signalling pathway gene list based on molecular signatures database (MSigDB) v7.4. (B) Dot plot showing significantly over-represented KEGG pathway terms that were defined from MSigDB v7.4 using the 3,207 DEGs with p < 0.05. (C) Dot plot showing significantly over-represented oncogenic pathway terms that were defined from MSigDB v7.4 using the 3,207 DEGs with p < 0.05. (D) Network diagram representing the mTOR signalling events mediated by PIK3CA, PIK3CB and EIF4B using STRING 2 weighted correlation network analysis (WGCNA). Asterisks represent statistically significant levels of gene differences between control and irradiated samples (p< 0.05) calculated using Student’s t test. NS, non-significant; FC, fold change.