TABLE 2.
Metal ion and inhibitor effects on the activity of the Fpp1 protease
| Compound(s) (concn [mM]) | Caseinolytic activity (%) |
|---|---|
| None | 100 |
| CaCl2 (0.01) | 100.6 ± 1.2 |
| CaCl2 (0.1) | 100.1 ± 1 |
| CaCl2 (0.5) | 146.8 ± 2.4 |
| CaCl2 (1) | 297.6 ± 5.3 |
| CaCl2 (5) | 301.6 ± 5.2 |
| CaCl2 (10) | 191.5 ± 3.6 |
| MgCl2 (1) | 99.2 ± 0.9 |
| MgCl2 (10) | 88.5 ± 1.8 |
| ZnCl2 (0.005) | 94.6 ± 0.5 |
| ZnCl2 (0.01) | 97.7 ± 2.0 |
| ZnCl2 (0.1) | 94.6 ± 0.7 |
| ZnCl2 (1) | 84 ± 1.5 |
| MnCl2 (1) | 117.7 ± 2.1 |
| MnCl2 (10) | 21.5 ± 3.8 |
| SrCl2 (1) | 250.8 ± 4.9 |
| SrCl2 (5) | 258.5 ± 5.2 |
| BaCl2 (1) | 172.6 ± 3.6 |
| BaCl2 (5) | 264.4 ± 5.8 |
| Phenylmethysulfonyl fluoride (1) | 97.9 ± 2.3 |
| 1,10-Phenanthroline (1) | 4.2 ± 1.5 |
| Dithiothreitol (1) | 78.8 ± 6 |
| Dithiothreitol (10) | 19.4 ± 1.4 |
| EDTA (1) | 18.5 ± 0.5 |
| EDTA (10) | 9.1 ± 1 |
| EGTA (1) | 12.3 ± 3 |
| EGTA (10) | 8.7 ± 1.2 |
| Zincov (1) | 101.1 ± 0.3 |
| EGTA (1) + CaCl2 (5) | 74.4 ± 3.2 |
| EGTA (1) + BaCl2 (5) | 70.5 ± 4.7 |
| EGTA (1) + SrCl2 (5) | 85.1 ± 5.3 |